Transposon mutagenesis of Bacteroides fragilis using a mariner transposon vector
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چکیده
منابع مشابه
A mariner transposon vector adapted for mutagenesis in oral streptococci
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Genetic analysis of Bacteroides fragilis (BF) is hindered because of the lack of efficient transposon mutagenesis methods. Here, we describe a simple method for transposon mutagenesis using EZ::TN5, a commercially available system that we optimized for use in BF638R. The modified EZ::TN5 transposon contains an Escherichia coli conditional origin of replication, a kanamycin resistance gene for E...
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Porphyromonas gingivalis is a gram-negative, black-pigmented, oral anaerobe strongly associated with adult periodontitis. Previous transposon mutagenesis studies with this organism were based on the Bacteroides transposon Tn4351. Characterization of Tn4351-disrupted genes by cloning has not been an efficient way to analyze large numbers of mutants and is further complicated by the high rate of ...
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ژورنال
عنوان ژورنال: Anaerobe
سال: 2013
ISSN: 1075-9964
DOI: 10.1016/j.anaerobe.2013.04.012